Life Sensors是一家為藥物發(fā)現和研究市場提供方法和建立可行性技術方法的生物技術公司。該公司為泛素和泛素蛋白酶體通路研究提供創(chuàng)新性的研究工具而占據世界。其原核和真核蛋白表達系統(tǒng)SUMOpro 和SUMOstar 已被世界范圍內的學術、政府研究機構和制藥、生物技術公司廣泛應用。

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SilenciX Ubiquitin Pathway Knockdown Cell Lines The first generation of RNAi tools, siRNA and basic plasmids expressing shRNA, reveal certain limitations and off-target effects. Furthermore, successful RNAi experiments currently involve significant optimization requiring additional resources. SilenciX® stable knockdown cell lines have been developed based on a unique siRNA delivery system developed by tebu-bio Laboratories* providing improved efficacy and stability of RNAi knockdown. Based on EBV-replication, this loss-of-function model ensures chromosome-like replication of the expression vector in a limited and constant number without integration in the genome. This eliminates off-target effects and saturation of the RNAi machinery resulting in specific and homogeneous knockdown of the desired target. LifeSensors is offering SilenciX®, gene-specific ready-to-use knock-down cell lines ‘silencing’ deubiquitylases （DUBs） and other proteins of interest, including DNA repair-related genes, providing invaluable tools for loss-of-function approaches. *SilenciX® is a registered trademark of tebu-bio; technology licensed from the CEA. SILENCIX® KNOCK-DOWN CELL LINES - DEUBIQUITYLASES （DUBS） SILENCIX® KNOCK-DOWN CELL LINES - DNA DAMAGE AND REPAIR SILENCIX® KNOCK-DOWN CUSTOM CELL LINES

Custom Ubiquitinated Peptides Synthesis of custom monoubiquitinated peptides designed from your protein of interest Choose the protein. . . Choose the ubiquitination site. . . We’ll deliver a monoubiquitinated peptide! LifeSensors can provide custom monubiquitinated peptides from the sequence of your choosing. lsopeptide-linked ubiquitinated peptide reagents are produced using peptide synthesis technologies. Stepwise solid-phase synthesis and fragment condensation / chemical ligation approaches are employed in the synthesis of the ubiquitinated peptides. By utilizing these complementary methodologies for peptide synthesis, a large variety of native and labelled 'isopeptide-linked' ubiquitinated peptides can be generated for a variety of applications including: •    DUB substrates for screening •    Antigens for generation of site-specific ubiquitin antibodies •    Structural studies of ubiquitin-binding domain recognition In contrast to traditional DUB substrates, like ubiquitin-AMC and ubiquitin-rhodamine, which measure cleavage of amide bonds, utilization of a monoubiquitinated peptide as a DUB substrate measures cleavage of an isopeptide bond and allows determination of the influence of sequence proximal to the ubiquitinated lysine on specificity. Profiling activity of a panel of DUBs against a monoubiquitinated histone H2B peptide and a monoubiquitinated PTEN peptide reveals interesting differences in specificity:   Assessment of DUB activity using monoubiquitinated peptides as a substrates. 2µM monoubiquitinated histone H2B peptide （red）, 2µM monoubiquitinated PTEN peptide （blue）, and 2µM ubiquitin-rhodamine were incubated with 50nM of each indicated DUB for 30 minutes at 37ºC. Isopeptidase cleavage of ubiquitin from the monoubiquitinated peptides was assessed by LC-MS and is represented as the percent substrate cleaved. Cleavage of ubiquitin-rhodamine was measured as the increase in rhodamine fluorescence at 535nm and is represented as the rate （RFU/sec） relative to USP2c. Monoubiquitinated PTEN and histone H2B peptides are available from stock. Length: peptides of almost any length can be synthesized Quantity: µg to gram quantities Purity: choose purity levels from 80-98% Fluorescent modifications: examples include fluorescein, TAMRA, AlexaFluor®, or FRET pairs Affinity tags: examples include biotin, His6, FLAG®, V5 Ubiquitin options: peptides can be synthesized with wild-type or mutant ubiquitins, including lysine to arginine substitutions or unnatural amino acids Contact us at info@lifesensors.com or call 610-644-8845 for a quotation or more information.